VOLTEC

Selection of SPE phases: The scheme below is meant as a first guide to the application of CHROMABOND columns of CHROMAFIX cartridges and considers mostly primary interactions. It is only valid for samples with molecular weights below 5000 Dalton to avoid size exclusion effects which might block the pores of the SPE silica. The solvents recommend for elution should also be considered as a first selection. Applicability of other solvents or solvent mixtures is determined by the polarity required for a separation.	Polarity nonpolar	Solvent	Miscibility with water
		Hexane	No
		Isooctane	No
		Petroleum ether	No
		Cyclohexane	No
		Carbon tetrachloridee	No
		Methylene chloride	NO
		Tetrahydrofuran	Yes
		Diethyl ether	No
		Ethyl acetate	Poor
		Acetone	Yes
		Acetonitrile	Yes
		Isoproanol	Yes
		Methanol	Yes
		Water	Yes
	Polar	Acetic acid	Yes

Concise selection guide for SPE phases and solvents.