E S Industries
Description of Traditional Reverse Phases

Phase

Phase Description

Preferred Application

TMS (C1)

Tri-Methyl

Low-polarity, separation of mixtures with differing Retention.

C2

Dimethyl

Retention times on this phase are much shorter Than other RP phases

C3

n-Propyl

Same As C2, but more stable

C4

n-butyl

Separation of peptides and proteins

C6

n-hexyl

Even Coverage, very suitable for mobile phase Additives

MC-CC6 endcapped

Cyclohexyl

Cyclohexyl but endcapped to making it more Suitable for polar solutes. Extremely useful for Herbicides and other related compounds.

C8

n-octyl

Reversed phase and ion-pairing chromatography. Moderately to highly polar (Water Soluble) Compounds such as small peptides, proteins, Steriods, nucleosides, and pharamaceuticals.

MC-8

n-octyl

Same as C8 but endcapped to make it more Suitable for polar solutes.

C18

Octadecyl

Nonpolar to moderately polar compounds such As fatty acids, clycerides, polyaromatic Hydrocarbons, esters, fat-soluble vitamins, Steroids, prostaglandins and pharmaceuticals,.

MC-18 endcapped

Octadecyl

Same as C18 but endcapped to make it more Suitable for acids andbases.

AP

Alkyl Phenyl

Moderately polar compounds. Retention Characteristics are similar to C8 packing, but with Different selectivity for polycyclic aromatic Hydrocarbons, polar aromatics and fatty acids.

C8-BD

Disopropyl/Octyl

Same as C8, but highly base deactivated using Steric shielding.

C18-BD

Diisorpropyl/Octyl

Same As C18, but highlybase deactivated using Steric shielding

P-BD

Dissopropyl/Phenyl

Same as AP, but highly base deactivated using Steric shielding.

CN

Cyano

Offers different selectivity than C8. C18, and Phenyl

CN-BD

Dissopropyl/Cyano

Same as CN, but highly base deactivated using Steric shielding.

CN-HS

Cyano

Same as CN, but higher surface area.

C18-Al

Octadecyl with amide linkage

Use for the Separation of weak acids.

D/RP

Diol

Diol for reverse phase

A/RP

Amino

NH2 amine for reverse phase, used for sugars

DA/RP

Diamine

Diamine for reverse phase

TA/RP

Triamine

Triamine for reverse phase.

 

ES industries has developed wide range of Chromegabond reverse phase columns to provide the means of separating a board range of compounds.

These phases are manufactured using established procedures and have been produced for a number of years to provide the chromatographer/QC chemist with continuous stream of highly reproducible columns. Chromegabond Reverse Phase Columns provide superior, reproducible separations and long column life with excellent peak symmetry. We have listed only the most popular sizes, however, all of the listed phases are available in any column dimensions that maybe required.

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