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New
technology results in durable, permanently bonded
phases
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Greatly
improves reproducibility
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Allows
precise control of EOF
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Positive
and negative walls available
|
Microcap® capillaries represent a significant advance
in CE technology. Microcap® capillaries contain
strong anion- and cation-exchange-coated microspheres permanently
bonded to the interior walls.
There are numerous benefits to modification of capillary
walls, including control of EOF and prevention of analyte
adsorption. Before Microcap® technology, capillary
wall modification was achieved using non- permanent, dynamic
coatings, which have several drawbacks. Non-permanent coatings
limit choice of electrolytes and are stripped from the capillary
wall with repeated use. The sample matrix often disturbs
dynamic coatings which leads to poor reproducibility. Worse
yet, dynamic coatings can precipitate, destroying the capillary.
Microcap® capillaries overcome the disadvantages
of dynamic coatings. Microcap’s ionic spheres cannot be
stripped from the capillary, even after repeated use at
extended pH ranges and varied electrolyte ionic strength.
The graph above shows the relative independence of EOF and
pH, resulting in more rugged methods and more flexibility
in developing separation conditions. The wall coating composition
is sample-independent so reproducibility is greatly improved,
and of course, permanently-bonded coatings cannot not “precipitate.”
Microcap capillaries have been applied to a wide range of
separation problems, including analysis of proteins, pharmaceuticals,
and inorganic ions. Capillaries are available in any length
with internal diameters of 75µ or 50µ. Common dimensions
are shown in the ordering information section below.
Please specify placement of detection window when
ordering. |
