VOLTEC

MetaChem MetaCarb ™

Carbohydrate, Organic Acid, and

Alcohol Analysis

The MetaCarb™ line of carbohydrate columns has recently been expanded to provide both a Bio-Rad Aminex® equivalent series as well as a series of very high resolution columns for applications requiring higher performance.

The Bio Rad Aminex equivalent series include the MetaCarb 87C, 87H, and 87P. These columns contain sulfonated polystyrene resins in the Calcium (87C), Hydrogen (87H), and Lead (87P) forms. These columns provide a wide range of selectivities for carbohydrate and organic analysis. Column selectivities are virtually identical to those provided by Bio Rad Aminex columns, while providing outstanding reproducibility and durability. The MetaCarb 87 series is based on an 8-10µ, 8% cross linked styrene divinylbenzene (SDVB) resin. MetaCarb 87 series columns are very rugged, operate at low back pressure, and provide outstanding column lifetime. Every 87 series column is provided with the following guarantee: If you are not completely satisfied with the performance of any MetaCarb column simply return it for a full refund. Period!!

For chemists searching for columns with increased resolution, the MetaCarb 67C columns and the MetaCarb Plus series are recommended. The MetaCarb Plus series contains a specially formulated styrene divinylbenzene resin modified by an “enhanced sulfonation” bonding technique to provide columns with higher resolving power than the standard 87 series.

The MetaCarb 67C column contains a 9µ, 6% cross linked sulfonated SDVB in the calcium form. This column provides excellent resolution of monosaccharides and sugar alcohols, particularly mannitol and sorbitol, and has set the standard for column reproducibility. The MetaCarb Ca Plus column contains an 8µ, highly cross-linked, sulfonated SDVB resin in the calcium form. This column is recommended for analysis of monosaccharides, disaccharides, and sugar alcohols. The column is widely used in the food and beverage industries for analysis of sweeteners, corn and cane sugars, fruit juices, soft drinks, beer, and dairy products. The column is also used to analyze sugar substitutes such as maltitol, xylitol, palatinitol, lactitol, mannitol, and sorbitol. Simple eluents such as deionized water, a dilute solution of calcium EDTA in deionized water, or a simple water: organic mixture are used with the MetaCarb Ca Plus column. The primary separation mechanism is ligand exchange as the calcium groups interact with hydroxyl groups in the sample. The MetaCarb Ca Plus column can be regenerated with a solution of calcium nitrate to extend column life.

The MetaCarb H Plus column contains an 8µ, highly cross-linked SDVB resin in the hydrogen form. This column is useful for analysis of samples containing monosaccharides in combination with organic acids, alcohols, ketones, fatty acids, or other neutral compounds. (Disaccharides are subject to acid hydrolysis on all Hydrogen-form columns). The primary separation mechanisms include ligand-exchange, ion-exclusion, and adsorption.

The MetaCarb Pb Plus column contains an 8µ highly cross-linked, sulfonated SDVB resin in the lead ionic form. This column is designed for analysis of monosaccharides and sugar alcohols. Cellobiose, glucose, xylose, arabinose, mannose and rhamnose, as well as other pentoses and hexoses found in cellulose products are well separated on this column. The MetaCarb Pb Plus is generally used with a deionized water eluent.

The MetaCarb USP L-19 Carbohydrate column is recommended for sugar alcohol analysis according to USP procedures. Sugar alcohols like sorbitol and mannitol are nicely resolved using simple isocratic eluents at low temperatures.

Also available are columns for oligosaccharide analysis (MetaCarb 47N), and columns in the sodium (MetaCarb 87N) and potassium (MetaCarb 87K) forms for different carbohydrate selectivities.

Three columns are available for analysis of amino acids on the Beckman 6300/7300 and System Gold amino acid analyzers.

Column	Typical Application	Column Size (mm)	Ionic Form	Guard Cartridges	Analytical Column
Column	Typical Application	Column Size (mm)	Ionic Form	20 x 4mm, 2/pk $130	Order No.	Price

MetaCarb 87C	Disaccharides - Sucrose, Maltose	300 x 7.8	Calcium	5200-GC	5200	$ 769
MetaCarb Ca Plus	Monosaccharides, Sugar Alcohols	300 x 7.8	Calcium	5205-GC	5205	$ 799
MetaCarb 67C	Monosaccharides, Sugar Alcohols	300 x 6.5	Calcium	5235-GC	5235	$ 779

MetaCarb 87H	Sugar/Organic Acid Mixtures	300 x 7.8	Hydrogen	5210-GC	5210	$ 749
MetaCarb H Plus	Sugar/Organic Acid Mixtures, Alcohols	300 x 7.8	Hydrogen	5215-GC	5215	$ 749
MetaCarb 67H	Sugars and Organic Acids	300 x 6.5	Hydrogen	5244-GC	5244	$ 749

MetaCarb 87P	Xylose, Mannose, Higher Saccharides	300 x 7.8	Lead	5220-GC	5220	$ 835
MetaCarb Pb Plus	Monosaccharides, Oligosaccharides	300 x 7.8	Lead	5241-GC	5241	$ 879

MetaCarb 87N	Monosaccharides, Oligosaccharides	300 x 7.8	Sodium	5041-GC	5041	$ 769
MetaCarb 87K	Beet Sugar, Cane Sugar, Corn Syrup	300 x 7.8	Potassium	5095-GC	5095	$ 769
MetaCarb Oligo	Oligosaccharides	300 x 7.8	Sodium	5238-GC	5238	$ 779
MetaCarb PAD	Amperometric Detection of Sugars	150 x 6.5	Sodium	5232-GC	5232	$ 649

USP L19	Sugar Alcohols	250 x 4.0	Calcium	5092-GC	5092	$ 499
USP L17		100 x 7.8	Hydrogen	5059-GC	5059	$ 599

MetAmino Li	Lithium column for Beckman 6300/7300	100 x 4.0	Lithium	5250-GC	5250	$ 1695
MetAmino Na	Sodium column for Beckman 6300/7300	120 x 4.0	Sodium	5252-GC	5252	$ 1695
MetAmino Gold	Lithium column for Beckman System Gold	120 x 4.0	Sodium	5254-GC	5254	$ 1595

Universal Guard Cartridge Holder		24 x 4.0		5204 @ $ 105

Mechanism of Carbohydrate Separation on Sulfonated Resins
	Carbohydrates are separated by ion-exchange, ligand-exchange, and ion-exclusion mechanisms. In this example, sugar 1 would be retained longer than sugar 2 because it has more hydroxyls available for simultaneous interaction with the stationary phase metal ion.